vitro transcription reaction Search Results


quick coupled sp6 in vitro transcription and translation (tnt) reaction  (Promega)
90
Promega quick coupled sp6 in vitro transcription and translation (tnt) reaction
Quick Coupled Sp6 In Vitro Transcription And Translation (Tnt) Reaction, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/quick+coupled+sp6+in+vitro+transcription+and+translation++tnt++reaction/us07063946-1137-18-21
Average 90 stars, based on 1 article reviews
quick coupled sp6 in vitro transcription and translation (tnt) reaction - by Bioz Stars, 2026-10
90/100 stars
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in vitro transcription reactions  (Promega)
90
Promega in vitro transcription reactions
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Transcription Reactions, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/in+vitro+transcription+reactions/pmc04081000-265-9-12
Average 90 stars, based on 1 article reviews
in vitro transcription reactions - by Bioz Stars, 2026-10
90/100 stars
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in vitro translation promega tnt  (Promega)
90
Promega in vitro translation promega tnt
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Translation Promega Tnt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/in+vitro+transcription+translation+reactions+promega+tnt/us07115711-278-13-15
Average 90 stars, based on 1 article reviews
in vitro translation promega tnt - by Bioz Stars, 2026-10
90/100 stars
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proteins produced by in vitro coupled transcription-translation reactions tnt kit  (Promega)
90
Promega proteins produced by in vitro coupled transcription-translation reactions tnt kit
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
Proteins Produced By In Vitro Coupled Transcription Translation Reactions Tnt Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/proteins+produced+by+in+vitro+coupled+transcription+translation+reactions+tnt+kit/10__1128_slash_mcb__26__10__3942___3954__2006-59-18-28
Average 90 stars, based on 1 article reviews
proteins produced by in vitro coupled transcription-translation reactions tnt kit - by Bioz Stars, 2026-10
90/100 stars
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bacterial linear template s30 coupled in vitro transcription/translation reaction kit  (Promega)
90
Promega bacterial linear template s30 coupled in vitro transcription/translation reaction kit
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
Bacterial Linear Template S30 Coupled In Vitro Transcription/Translation Reaction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/bacterial+linear+template+s30+coupled+in+vitro+transcription+translation+reaction+kit/us08679781-425-16-9
Average 90 stars, based on 1 article reviews
bacterial linear template s30 coupled in vitro transcription/translation reaction kit - by Bioz Stars, 2026-10
90/100 stars
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biotin-labeled in vitro transcription reaction  (Bioarray Inc)
90
Bioarray Inc biotin-labeled in vitro transcription reaction
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
Biotin Labeled In Vitro Transcription Reaction, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/biotin+labeled+in+vitro+transcription+reaction/pmc00186645-401-15-21
Average 90 stars, based on 1 article reviews
biotin-labeled in vitro transcription reaction - by Bioz Stars, 2026-10
90/100 stars
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tnt quick coupled in vitro transcription/translation reaction product  (Promega)
90
Promega tnt quick coupled in vitro transcription/translation reaction product
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
Tnt Quick Coupled In Vitro Transcription/Translation Reaction Product, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/tnt+quick+coupled+in+vitro+transcription+translation+reaction+product/pmc04672336-190-13-12
Average 90 stars, based on 1 article reviews
tnt quick coupled in vitro transcription/translation reaction product - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
dsrna from in vitro transcription reactions  (Promega)
90
Promega dsrna from in vitro transcription reactions
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
Dsrna From In Vitro Transcription Reactions, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/dsrna+from+in+vitro+transcription+reactions/pmc03177309-51-3-10
Average 90 stars, based on 1 article reviews
dsrna from in vitro transcription reactions - by Bioz Stars, 2026-10
90/100 stars
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in-vitro transcription reaction mix ribomax  (Promega)
90
Promega in-vitro transcription reaction mix ribomax
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Transcription Reaction Mix Ribomax, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/in+vitro+transcription+reaction+mix+ribomax/pm25329993-200-15-20
Average 90 stars, based on 1 article reviews
in-vitro transcription reaction mix ribomax - by Bioz Stars, 2026-10
90/100 stars
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in vitro transcription/translation (itt) reaction mixture  (Isogenica Limited)
90
Isogenica Limited in vitro transcription/translation (itt) reaction mixture
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Transcription/Translation (Itt) Reaction Mixture, supplied by Isogenica Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/in+vitro+transcription+translation++itt++reaction+mixture/pmc06341108-260-22-27
Average 90 stars, based on 1 article reviews
in vitro transcription/translation (itt) reaction mixture - by Bioz Stars, 2026-10
90/100 stars
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in vitro transcription/translation reaction using the manufacturer’s protocol  (Promega)
90
Promega in vitro transcription/translation reaction using the manufacturer’s protocol
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Transcription/Translation Reaction Using The Manufacturer’s Protocol, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vitro+transcription+reaction/in+vitro+transcription+translation+reaction+using+the+manufacturer%E2%80%99s+protocol/pm10766840-58-22-29
Average 90 stars, based on 1 article reviews
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Promega in vitro transcription and translation mix
(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 <t>transcription</t> factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.
In Vitro Transcription And Translation Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 transcription factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.

Journal: PLoS Genetics

Article Title: zic-1 Expression in Planarian Neoblasts after Injury Controls Anterior Pole Regeneration

doi: 10.1371/journal.pgen.1004452

Figure Lengend Snippet: (A) Measurement of expression changes in X1 neoblasts due to treatment with zic-1 versus control dsRNA. Trunk fragments were macerated 5 minutes (0 days), 24 hours (1 day) and 48 hours (2 days) after head and tail amputation, and X1 cells were isolated from 3 independent biological replicates of 10 worms each. qPCR examined putative expression changes in a cohort of 15 transcription factors and signaling molecules that mark neoblast subpopulations induced by regeneration. Cartoon depicts experimental design. Heat map shows log 2 fold-changes in gene expression for the indicated genes in zic-1(RNAi) X1 neoblasts versus controls averaged from three biological replicates for each condition. Blue color indicates downregulation and yellow indicates upregulation, due to zic-1 RNAi. (B–D) Double FISH to detect expression of zic-1 , foxD , smedwi-1 and/or notum with nuclei counterstained with Hoechst (gray). (B) In situ hybridization probing to detect notum (magenta) and smedwi-1 (green) to detect notum+smedwi-1+ cells. (B, top) Representative image of notum+smedwi-1 + cells, (B, bottom) quantification of notum+smedwi-1+ cells. zic-1(RNAi) reduced numbers of notum+ neoblasts by 48 hours and smedwi-1+ cells were more numerous in anterior than posterior. (C) foxD (magenta) expression in smedwi-1+ (green) neoblasts 24 hours after injury was not eliminated due to zic-1 RNAi. Bottom, quantification of foxD+smedwi-1+ cells from control and zic-1(RNAi) animals fixed 24 hours after head and tail amputation and probed by double FISH. foxD+smedwi-1+ cells were produced in equal numbers at anterior- versus posterior-facing wound sites and were not reduced by zic-1 inhibition (control animals, 29±1 foxD+smedwi-1+ cells in anterior- versus 32±4 in posterior-facing wounds; zic-1 RNAi animals, 19±5 foxD+smedwi-1+ cells in anterior- versus 26±6 in posterior-facing wounds, n = 3 worms each). (D) foxD (green) is expressed in a subpopulation of zic-1+ cells (magenta, 3.4±1.8% of 420 zic-1 + cells were foxD +, n = 5 worms) by 24 hours. Yellow arrow indicates double positive cell (also inset), and white arrows indicate zic-1+ cells. Cartoons show surgeries and zoomed areas. Error bars standard deviations, asterisks p-value<0.05 by t-test. Bars, 75 microns.

Article Snippet: For RNAi by injection, dsRNA was synthesized from in vitro transcription reactions (Promega) using PCR templates with flanking T7 sequences (Denville), purified by phenol extraction and ethanol precipitation, resuspended in RNase-free water and annealed at 65°C for 10 minutes, 37°C for 20–30 minutes, then on ice for at least 10 minutes. dsRNA corresponding to C. elegans unc-22 , a gene not present in the planarian genome, served as a negative control.

Techniques: Expressing, Control, Isolation, Gene Expression, In Situ Hybridization, Produced, Inhibition